The NeoNat SCID SMA is a Real-Time PCR assay for screening Severe Combined Immunodeficiency (SCID) Syndrome and Spinal Muscular Atrophy (SMA) in newborns DNA from Dried Blood Spot (DBS) samples.
overview
Greater flexibility with separate multiplexing of SCID and SMAfrom one kit
Kit contains all the reagents and components to perform DNA extraction from DBS
Ready to use reaction mix and calibrators preloaded plates enable convenience for users
Four levels of DBS controls with defined target gene copies per μL
Compatible with multiple qPCR instruments
Short turnaround time of ~120 mins from DNA extraction to result analysis
Technical Specifications
Assay Description
NeoNat SCIDSMA Real time PCR kit is based on 5’ nuclease technique
Screens T-cell receptor excision circle (TREC) / Kappa deleting recombination excision circle (KREC) for SCID and Survival Motor Neuron 1 (SMN1) & Survival Motor Neuron 2 (SMN2) for SMA. The ¬-globin gene serves as an internal control
Performance
The assay demonstrates excellent performance with 100% sensitivity and specificity for all the samples tested
The analytical sensitivity shows a limit of detection of <5.0 copies/L for TREC
& KREC and <2.5 copies/μL for SMN1 & SMN2
Compatibility
Kit compatible with most real-time PCR instruments in the market with at least three measurement channels (FAM, VIC/or HEX, & CY5)
Developed and validated with Biorad CFX 96 and Thermofisher Quantstudio 5 & 6.
