Next-generation sequencing (NGS) has revolutionized the field of genomics and is increasingly being used for disease diagnosis. NGS can detect even low levels of genetic mutations or variations, which may not be detectable by traditional sequencing methods. This makes it highly useful for identifying rare genetic disorders and diagnosing diseases at an early stage. NGS can analyze large amounts of DNA in a short amount of time, making it faster than traditional sequencing methods.The most important application of NGS appears in the screening for multiple genes. NGS can screen for multiple genes simultaneously, which is useful for identifying genetic predispositions to diseases or for analysing multiple genes in cancer patients to guide treatment decisions.