The “MTBSure - Mycobacterium tuberculosis Real-Time PCR Kit” is a molecular diagnostic technology leveraging fluorescent probe-based methods for the rapid and accurate detection of Mycobacterium tuberculosis DNA. Built upon the principles of PCR, this real-time variant incorporates specialized fluorescent probes into the reaction mixture. These probes emit a signal during DNA amplification, allowing continuous monitoring of the process in real time. The specific region of the MTB genome is targeted and amplified, and the fluorescence signals are quantitatively analyzed. MTB RT-PCR exhibits high sensitivity and specificity, particularly adept at detecting TB in cases with low bacterial loads or extrapulmonary manifestations.