The JAK family of non-receptor tyrosine kinases includes JAK1, JAK2, JAK3, and TYK2. Growth factors and cytokines can regulate gene transcription through JAK-dependent activation of signal transducer and activator of transcription (STAT). The JAK-STAT signal transduction pathway regulates cell proliferation, differentiation, apoptosis, and immune regulation. The Jak2 gene is associated with a genetic mutation known as the JAK2 V617F mutation, which is primarily associated with myeloproliferative disorders (MPD), including polycythemia vera (PV), essential thrombocythemia, and idiopathic myelofibrosis. JAK2 mutation is the main diagnostic indicator of MPD. The “JAK-2Sure: JAK2-V617F Mutation Detection Real Time PCR Kit” is a molecular diagnostic technology utilizing the Taqman fluorescent probe-based chemistry for the amplification of conserved regions of JAK2 mutant DNA. The amplified product is detected by the generation of fluorescent signals from target specific fluorescence labelled probes.