COVIDsure DIRECT

Multiplex RT-PCR kit with Extraction Free Technology

overview

The COVIDsure DIRECT is a multiplex RT-PCR Kit for rapid qualitative in vitro detection of SARS-CoV-2 (COVID-19) directly from the VTM samples without RNA extraction from respiratory specimens (nasopharyngeal swab, oropharyngeal swab) of suspected cases in COVID-19 disease. The kit is supplied with RNA process buffer (RPB) which can be used for RNA sample preparation without extensive RNA extraction process. This kit can also detect SARS-CoV-2 virus from the RNA extracted from the VTM samples from suspected COVID-19 patients.

Technology

Multiplex RT-PCR kit with Extraction Free Technology

Extraction Free technology based on Magic buffer (Provisional Patent Filed).
Tedious and time-consuming RNA extraction process is replaced with one-step easy technology using RNA processing buffer (RPB).
Cost of RNA extraction kit and instruments is saved.
Simultaneous detection of three SARS-CoV-2 specific genes ORF1ab, N gene and S gene besides human internal control gene.
*Kit enables the in-direct detection of SARS-CoV-2 variants, Omicron, Alpha B 1.1.7, Eta B.1.525 by S gene target failure mechanism (SGTF).
Low virus load detection: < 5 copies/ µl
Works equally well on VTM as well as extracted RNA.
Highly Specific: 100% Specificity. No detectable cross reactivity with most human Coronavirus strains, respiratory pathogens, etc.
Utmost Sensitivity: 100% Sensitivity. Wider range of Covid-19 strains covered to detect most mutations (mutation variants)
Timesaving Approach: Processing time is approximately 45 mins - 60 mins

*Indirect detection of SARS-CoV-2 variants by S Gene must be verified by sequencing.

COVIDsure DIRECT

overview

Technology

Test Procedure