NeoMass CAH
Second-Tier LC-MS/MS Kit for Confirmation of Congenital Adrenal Hyperplasia (CAH) Accurate confirmation. Reduced false positives. Reliable newborn ...
Overview
NeoMass™ CAH is a second-tier LC-MS/MS-based diagnostic kit designed for the confirmation of Congenital Adrenal Hyperplasia (CAH) from dried blood spot samples. CAH represents a group of inherited disorders that disrupt normal cortisol and aldosterone synthesis due to enzyme deficiencies—most commonly 21-hydroxylase deficiency, accounting for about 95% of all cases. This leads to excess accumulation of steroid hormone precursors and elevated androgen production, which can cause adrenal insufficiency and genital ambiguity in newborns. The NeoMass™ CAH kit enables simultaneous and specific measurement of key steroids associated with CAH using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). This advanced method significantly improves screening accuracy by minimizing false-positive results from primary immunoassays for 17-hydroxyprogesterone.
Features
- Comprehensive Steroid Profiling: Detects and quantifies key CAH-related steroids including Androstenedione, Cortisol, 11-Deoxycortisol, 21-Deoxycortisol, 17-Hydroxyprogesterone, and 21-Hydroxyprogesterone.
- Fast Sample Preparation: Simple DBS workflow with preparation under 45 minutes using reaction tubes or 96-well plates.
- Isotopically Labelled Standards: Internal standards for every analyte ensure accuracy and traceability.
- Validated Calibrators and Controls: Includes 8-level DBS calibrators and 3-level DBS controls meeting CLSI requirements.
- Efficient LC-MS/MS Performance: Achieves clear chromatographic separation within 10 minutes, including challenging isobars.
- Broad Instrument Compatibility: Works with mid- and high-sensitivity LC-MS/MS instruments across major platforms.
- Modular and Flexible: Individually available components for seamless workflow integration.
Technical Specifications
- Analytes : Androstenedione, Cortisol, 11-Deoxycortisol, 21-Deoxycortisol, 17-Hydroxyprogesterone, 21-Hydroxyprogesterone.
- Sample Type : Dried Blood Spot (1 DBS disc).
- Sample Preparation Time : Less than 45 minutes.
- Chromatographic Run Time : Approximately 10 minutes.
- Calibration Levels : 8-level DBS calibrators.
- Control Levels : 3-level DBS controls.
- Internal Standards : Isotopically labelled standards for all analytes.
- Compatibility : Compatible with most mid- and high-sensitivity LC-MS/MS instruments.
Benefits
- Enhances accuracy and specificity in newborn screening for CAH.
- Reduces false-positive rates, improving clinical decision confidence.
- Provides a robust and standardized workflow for confirmatory testing.
- Minimizes manual handling with simplified sample preparation.
- Ensures reliable, reproducible results through isotopically labelled internal standards.