NeoNat SCID Real Time PCR
A Real-Time PCR-based neonatal screening solution for detecting TREC and KREC levels from dried blood spot samples — enabling early identification ...
Overview
Severe Combined Immunodeficiency (SCID) represents a group of rare, inherited disorders caused by genetic mutations affecting immune cell development and function. Early detection is critical to prevent life-threatening infections and initiate timely treatment.
Labsystems Diagnostics NeoNat SCID Real Time PCR Kit enables semi-quantitative determination of T-cell Receptor Excision Circles (TREC) and Kappa-deleting Recombination Excision Circles (KREC) from newborn DNA in dried blood spot (DBS) samples.
This assay offers a reliable, high-throughput method for newborn SCID screening with precise differentiation of immune function abnormalities.
Features
- Direct DNA extraction from dried blood spot samples
- Ready-to-use optimized reaction mix for simplified workflow
- Includes DBS calibrators and controls for accurate quantification
- Detects TREC, KREC, and globin in a single PCR reaction
- Based on 5’ nuclease amplification technique
- Compatible with a wide range of Real-Time PCR instruments
- 100% clinical sensitivity and specificity validated
- Designed for reproducibility and robust performance across laboratories
Technical Specifications
- Target Genes : TREC, KREC, globin (internal control)
- Sample Type : Dried Blood Spot (DBS)
- Technology : 5’ nuclease Real-Time PCR
- Controls : DBS calibrators and internal controls included
- Compatibility : Suitable for most Real-Time PCR systems
- Sensitivity/Specificity : 100% clinical validation