High-performance liquid chromatography is a chromatographic technique that is performed to separate a mixture of compounds in analytical chemistry and biochemistry techniques, and it helps to identify and quantify the individual components of the target mixture present in the sample. High-performance liquid chromatography is a chromatographic technique that is performed to separate a mixture of compounds in analytical chemistry and biochemistry techniques, and it helps to identify and quantify the individual components of the target mixture present in the sample.