Best in Precision ELISA Tests by Trivitron Healthcare
ELISA (Enzyme Linked Immunosorbent Assay) kits are commonly used for research and diagnosis. They detect and quantify biological molecules such as proteins, antibodies, hormones, and antigens with a high degree of sensitivity, specificity, and precision.
ELISA kits detect and evaluate the presence and concentration of a target biomolecule in a sample by utilizing specific antibodies. ELISA is based on the principle of binding the target molecule to an immobilized capture antibody, followed by detection with a labeled antibody that recognizes a different epitope on the target molecule. The label for detection may be generated by an enzyme that produces a colorimetric, fluorescent, or chemiluminescent signal. It is proportional to the target molecule concentration present in the sample.
ELISA kits have a multitude of benefits over Western blot, immune-histochemistry, and radio-immunoassay. It is a rapid, precise, and economical assay that requires only a small amount of sample and can be easily automated for high-throughput screening. Depending on the sensitivity of the assay and the target molecule, these kits provide a wide dynamic range of detection, from picograms to nanograms per milliliter.
These kits are used in a variety of diagnostic and research applications, including measuring the concentration of biomarkers in blood, serum, or urine samples for disease diagnosis, monitoring the efficacy of drug therapies, and detecting the presence of infectious agents or environmental toxins. Additionally, ELISA kits are utilized in basic research to examine the expression, localization, and function of proteins and other biomolecules in cells and tissues. Due to their high sensitivity, specificity, and adaptability, they have become important tools in biomedical research and clinical diagnostics.
ELISA Kits by Trivitron Healthcare are Used in Diseases Diagnosis
HIV Ag /Ab ELISA Test
HIV Ag/Ab ELISA Test is a high-quality diagnostic test designed to detect the presence of HIV-1 p24 antigen and antibodies to HIV-1 and HIV-2 in human serum or plasma. This kit utilizes reliable and sensitive sandwich immuno sorbent assay method technique for detection of HIV infection.
The kit has all the necessary components for performing the assay, including 96-well micro-titer plates coated with HIV-1 p24 antigen, HIV-1 and HIV-2 recombinant antigens, and HIV-1 gp41 antigen, as well as control reagents, wash buffer, conjugate buffer, and substrate solution.
The assay is performed by adding the sample and a diluent to the coated micro-titer wells, allowing them to incubate, and then washing away any unbound material. A conjugate solution containing Horseradish Peroxidase (HRP) is added to the wells, and after another incubation and wash step, a substrate solution is added. The color intensity is then measured, correlating with the presence or absence of HIV-1 p24 antigen and HIV-1 and HIV-2 antibodies in the sample.
The kit has a sensitivity of 99.8% and a specificity of 99.7%, making it an excellent tool for diagnosing HIV infection. The kit is CE-IVD certified and meets all the necessary quality standards for diagnostic tests.
HCV ELISA Test
HCV ELISA Test from Trivitron Healthcare is a high-quality diagnostic test designed to detect the presence of antibodies to the Hepatitis C virus (HCV) in human serum or plasma.
The kit includes all the necessary components for performing the assay, including 96-well micro-titer plates coated with HCV recombinant antigens and control reagents, wash buffer, conjugate buffer, and substrate solution.
HCV ELISA Test has a sensitivity of 99.9% and a specificity of 99.8%, making it an excellent tool for diagnosing HCV infection.
HBsAg ELISA Test
HBsAg ELISA Test is used to detect the presence of Hepatitis B surface antigen (HBsAg) in human serum or plasma samples. The kit consists of microplate wells coated with monoclonal antibodies specific to HBsAg. When the serum or plasma sample is added to the microplate, any HBsAg in the sample binds to the immobilized antibodies. The unbound materials are removed, and an enzyme-conjugated detection antibody is added.
This conjugate binds to the captured HBsAg, forming a sandwich complex. A substrate solution is then added, which reacts with the enzyme to produce a color change proportional to the amount of HBsAg in the sample.
HBsAg ELISA Test has high sensitivity and specificity, making it a reliable tool for diagnosing acute and chronic Hepatitis B infections. The kit’s sensitivity is 100%, and it can detect all positive samples, while the specificity is 99.5%, ensuring that false positives are minimized.
The kit is compatible with various ELISA plate readers and requires standard laboratory equipment for sample handling and analysis. The kit is designed for in vitro diagnostic use only and should be used by trained professionals in clinical laboratories or hospitals.
Syphilis Ab ELISA Test
It is a qualitative in-vitro diagnostic immunoassay for the detection of Treponema pallidum (Tp) antibodies in human serum or plasma. The kit is primarily intended for use by healthcare professionals for screening purposes.
Syphilis Ab ELISA Test is an enzyme-linked immunosorbent assay based on the double antigen sandwich principle for the detection of antibodies to Tp in human serum or plasma. The wells of a microplate are coated with recombinant Treponemal antigens. Other recombinant Treponemal antigens are conjugated with the peroxidase enzyme.
If antibodies against Treponemal antigens are present in the test sample, they will react with both the antigens coated on the micro-wells and the antigens conjugated to the peroxidase enzyme upon the addition of the conjugate. It forms a sandwich-like antigen-antibody-antigen enzyme complex. The enzyme activity is determined by adding a substrate that causes a blue color development. After stopping the enzyme-substrate reaction with a stop solution, the color changes from blue to yellow. A standard ELISA plate reader measures the absorbance at 450 nm (reference wavelength 620 nm).